We can track and see the production of single molecules, trace them and see how they assemble into structures.

I don't like saying 'no' to people, and I'm going to have to learn how to say 'no' more.

In essence, we're imaging the same cell for anywhere from forty to a hundred thousand times to create one of the movies that we see.

You need a continuous picture of how things are evolving, and not a slow series of snapshots where you don't know how frame A is related to frame B.

It's nice to be able to look at one protein, but life is driven by the interactions between proteins, so it's really essential to be able to see multiple proteins at a time to understand these interactions.

The question was, 'Is there a way of minimizing the amount of damage you're doing so that you can then study cells in a physiological manner while also studying them at high spatial and temporal resolution for a long time?'